Premium
Specific interaction between Sam68 and neuronal mRNAs: Implication for the activity‐dependent biosynthesis of elongation factor eEF1A
Author(s) -
Grange Julien,
Belly Agnès,
Dupas Stéphane,
Trembleau Alain,
Sadoul Rémy,
Goldberg Yves
Publication year - 2009
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21824
Subject(s) - eukaryotic translation elongation factor 1 alpha 1 , messenger rna , biology , hippocampal formation , immunoprecipitation , translation (biology) , microbiology and biotechnology , polysome , protein biosynthesis , neurite , ribosome , neuroscience , rna , gene , in vitro , biochemistry
In cultured hippocampal neurons and in adult brain, the splicing regulatory protein Sam68 is partially relocated to the somatodendritic domain and associates with dendritic polysomes. Transfer to the dendrites is activity‐dependent. We have investigated the repertoire of neuronal mRNAs to which Sam68 binds in vivo. By using coimmunoprecipitation and microarray screening techniques, Sam68 was found to associate with a number of plasticity‐related mRNA species, including Eef1a1, an activity‐responsive mRNA coding for translation elongation factor eEF1A. In cortical neuronal cultures, translation of the Eef1a1 mRNA was strongly induced by neuronal depolarisation and correlated with enhanced association of Sam68 with polysomal mRNAs. The possible function of Sam68 in Eef1a1 mRNA utilization was studied by expressing a dominant‐negative, cytoplasmic Sam68 mutant (GFP‐Sam68ΔC) in cultured hippocampal neurons. The level of eEF1A was lower in neurons expressing GFP‐Sam68ΔC than in control neurons, supporting the proposal that endogenous Sam68 may contribute to the translational efficiency of the Eef1a1 mRNA. These findings are discussed in the light of the complex, potentially crucial regulation of eEF1A biosynthesis during long‐term synaptic change. © 2008 Wiley‐Liss, Inc.