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Topology and patch‐clamp analysis of the sodium channel in relationship to the anti‐lipid a antibody in campylobacteriosis
Author(s) -
Usuki Seigo,
Nakatani Yoshihiko,
Taguchi Kyoji,
Fujita Tetsuhiro,
Tanabe Shinya,
Ustunomiya Iku,
Gu Yihua,
Cawthraw Shaun A.,
Newell Diane G.,
Pajaniappan Mohanasundari,
Thompson Stuart A.,
Ariga Toshio,
Yu Robert K.
Publication year - 2008
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21781
Subject(s) - antibody , ganglioside , epitope , campylobacter jejuni , campylobacteriosis , sodium channel , peptide , biology , tetrodotoxin , chemistry , biochemistry , microbiology and biotechnology , immunology , bacteria , endocrinology , sodium , genetics , organic chemistry
An infecting strain VLA2/18 of Campylobacter jejuni was obtained from an individual with campylobacteriosis and used to prepare chicken sera by experimental infection to investigate the role of serum anti‐ganglioside antibodies in Guillain‐Barré syndrome. Both sera of the patient and chicken contained anti‐ganglioside antibodies and anti‐Lipid A (anti‐Kdo2‐Lipid A) antibodies directed against the lipid A portion of the bacterial lipooligosaccharide. The anti‐Kdo2‐Lipid A activities inhibited voltage‐gated Na (Nav) channel of NSC‐34 cells in culture. We hypothesized that anti‐Kdo2‐Lipid A antibody acts on the functional inhibition of Nav1.4. To test this possibility, a rabbit peptide antibody (anti‐Nav1.4 pAb) against a 19‐mer peptide (KELKDNHILNHVGLTDGPR) on the α subunit of Nav1.4 was produced. Anti‐Nav1.4 pAb was cross‐reactive to Kdo2‐Lipid A. Anti‐Kdo2‐lipid A antibody activity in the chicken serum was tested for the Na + current inhibition in NSC‐34 cells in combination with μ‐Conotoxin and tetrodotoxin. Contrary to our expectations, the anti‐Kdo2‐Lipid A antibody activity was extended to Nav channels other than Nav1.4. By overlapping structural analysis, it was found that there might be multiple peptide epitopes containing certain dipeptides showing a structural similarity with v‐Lipid A. Thus, our study suggests the possibility that there are multiple epitopic peptides on the extracellular domains of Nav1.1 to 1.9, and some of them may represent target sites for anti‐Kdo2‐Lipid A antibody, to induce neurophysiological changes in GBS by disrupting the normal function of the Nav channels. © 2008 Wiley‐Liss, Inc.