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Effect of transferrin on hypomyelination induced by iron deficiency
Author(s) -
Badaracco M. E.,
Ortiz E. H.,
Soto E. F.,
Connor J.,
Pasquini J. M.
Publication year - 2008
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21709
Subject(s) - transferrin , myelin , antibody , myelinogenesis , ferritin , biology , endocrinology , immunohistochemistry , myelin associated glycoprotein , medicine , iron deficiency , myelin basic protein , immunology , microbiology and biotechnology , oligodendrocyte , biochemistry , anemia , central nervous system
We have used a model of iron deficiency in the rat to analyze the effects of a disruption in iron availability on oligodendroglial cell (OLGc) maturation and myelinogenesis and to explore the possible beneficial influence of an intracranial injection (ICI) of apotransferrin (aTf) at 3 days of age on this process. Studies carried out on postnatal days 17 and 24 showed that iron deficiency produced a decrease in myelin proteins and lipids at 24 days of age. Immunohistochemistry showed that in untreated iron‐deficient (ID) rats, the immunoreactivity of anti–adenomatous polyposis coli (APC) and anti‐MBP antibodies decreased markedly with reference to normal controls, whereas in ID rats treated with an ICI of aTf, the immunoreactivity of these markers increased. A similar situation occurred with the immunoreactivity of H‐ferritin. In primary OLGc cultures from ID rats, there was a high number of cells positive to the antibody against the polysialylated form of the cell surface glycoprotein NCAM (PSA‐NCAM) compared with in OLGc cultures prepared from normal controls or from ID animals treated with aTf. The number of MBP+ cells in cultures from ID rats increased after treatment with aTf. The presence of lipid rafts evaluated with a specific anti–protein prion cellular (PrPc) antibody showed a smaller number of PrPc‐positive structures in ID rat cultures. Treatment of the ID animals with a single ICI of aTf stimulated myelination, producing a significant correction in the different biochemical parameters affected by ID. © 2008 Wiley‐Liss, Inc.