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Ischemia‐induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damage
Author(s) -
Hwang In Koo,
Yoo KiYeon,
Kim Dae Won,
Kim So Young,
Park Jun Hong,
Ryoo Zae Young,
Kim Joon,
Choi Soo Young,
Won MooHo
Publication year - 2008
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21621
Subject(s) - neuroprotection , ischemia , tunel assay , hippocampal formation , biology , microbiology and biotechnology , chemistry , medicine , endocrinology , pharmacology , apoptosis , biochemistry
Some ribosomal proteins are important regulators of development and DNA repair. However, few studies have been conducted on ribosomal protein S3 (rpS3) in the ischemic hippocampus. In the present study, we investigated ischemia‐induced changes in rpS3 immunoreactivity, rpS3 mRNA, and protein levels in the hippocampal CA1 region of Mongolian gerbil after 5 min of transient forebrain ischemia. RpS3 immunoreactivity and its protein level were found to be significantly elevated at 6 hr after ischemia/reperfusion and then continuously decreased with time. RT‐PCR analysis also showed that rpS3 mRNA levels were significantly elevated in CA1 at 6 hr after transient ischemia. In addition, during the course of this study, we developed a delivery vector (Pep‐1) and its rpS3 fusion protein (Pep‐1–rpS3) to elucidate the role of rpS3 in ischemia‐induced damage. Pep‐1–rpS3 administration to ischemic animals significantly and dose dependently increased neuronal survival in the stratum pyramidale of CA1. Moreover, Pep‐1–rpS3 treatment reduced terminal deoxynucleotidyl dUTP nick‐end labeling‐positive CA1 pyramidal cell numbers in the stratum pyramidale. To elucidate how Pep‐1–rpS3 ameliorates ischemic damage, changes in 4‐hydroxy‐2‐nonnenal (HNE; an indicator of lipid peroxidation) immunoreactivity and protein levels were investigated. HNE levels and immunoreactivities in Pep‐1–rpS3‐treated ischemic animals were lower than in corresponding Pep‐1‐treated ischemic animals. These results indicate that rpS3 has a neuroprotective effect in the brain exposed to ischemia. © 2008 Wiley‐Liss, Inc.