Premium
Doublecortin‐expressing cells in the ischemic penumbra of a small‐vessel stroke
Author(s) -
Hua Rui,
Doucette Ron,
Walz Wolfgang
Publication year - 2008
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21546
Subject(s) - doublecortin , neocortex , lesion , subventricular zone , biology , corpus callosum , pathology , neural stem cell , rostral migratory stream , olfactory bulb , neurogenesis , neuroblast , glial fibrillary acidic protein , cortex (anatomy) , neuroscience , anatomy , hippocampal formation , immunohistochemistry , microbiology and biotechnology , central nervous system , medicine , stem cell , dentate gyrus
A cortical lesion was induced by disrupting the medium‐size pial vessels, which led to a cone‐shaped cortical lesion and turned into a fluid‐filled cavity surrounded by a glial acidic fibrillary protein‐positive (GFAP + ) glia limitans 21 days after injury. Therefore, it mimics conditions of lacunar infarctions, one of the most frequent human stroke pathologies. Doublecortin (DCX)‐positive cells were present in the neocortex and corpus callosum at the base of the lesion. The number of DCX‐positive cells in the corpus callosum was significantly increased from day 5 to day 14 compared with the control group. In contrast, there were no DCX‐positive cells in neocortex of control animals; the DCX‐positive cells appeared in the neocortex after lesioning and were maintained until 14 days postlesioning. Some of the DCX‐positive cells were also immunoreactive for βIII‐tubulin, another marker of immature neurons. They did not stain positively for markers of glia cells. The presence of these DCX‐positive cells near the lesion might indicate a migratory pathway for developing neuroblasts from the subventricular zone (SVZ) through the corpus callosum to the lesion. SVZ cells were labeled with a lipophilic molecule, 5‐ (and 6‐) carboxyfluorescein diacetate succinimidyl ester (CFSE) stereotaxical injections. Although rostral migratory stream and olfactory bulb were intensely labeled, no CFSE‐containing cells were found in the cortex beneath the lesion. These results do not support the idea that the DCX‐positive cells were originating from neural precursors of the SVZ, but they might be generated from local progenitor cells. © 2007 Wiley‐Liss, Inc.