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Oxidation of 14 C‐labeled compounds perfused by microdialysis in the brains of free‐moving rats
Author(s) -
Zielke H. Ronald,
Zielke Carol L.,
Baab Peter J.
Publication year - 2007
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21424
Subject(s) - microdialysis , glutamine , chemistry , glutamate receptor , citric acid cycle , biochemistry , oxidative phosphorylation , in vivo , metabolism , amino acid , extracellular , biology , receptor , microbiology and biotechnology
The oxidative capacity of the brain for alternate substrates, glucose, lactate, pyruvate, acetate, glutamate, and glutamine was determined by using microdialysis to infuse 14 C‐labeled compounds into the interstitial fluid of adult rat brain and by collecting the brain‐generated 14 CO 2 from the dialysis eluate. All compounds were readily oxidized. The recovery of 14 CO 2 was enhanced for those compounds metabolically close to entry into the TCA cycle or known to have a low interstitial concentration. Two compounds, pyruvate and lactate, demonstrated reciprocal competition when added as nonradioactive competitors. Oxidation of two amino acids, 14 C‐glutamate and 14 C‐glutamine, was stimulated by the addition of nonradioactive acetate and pyruvate. α‐Cyano‐4‐hydroxycinnamate decreased 14 C‐lactate and 14 C‐pyruvate oxidation, consistent with the transport of both compounds via a monocarboxylate transporter. The results of this in vivo study support the results of previous in vitro studies that showed that a wide range of compounds formed from glucose in the brain are also oxidized in the brain for energy production. © 2007 Wiley‐Liss, Inc.