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Enhanced neuronal differentiation in a three‐dimensional collagen‐hyaluronan matrix
Author(s) -
Brännvall K.,
Bergman K.,
Wallenquist U.,
Svahn S.,
Bowden T.,
Hilborn J.,
ForsbergNilsson K.
Publication year - 2007
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21358
Subject(s) - microbiology and biotechnology , nestin , embryonic stem cell , synapsin i , cellular differentiation , neurite , progenitor cell , biology , basic fibroblast growth factor , stem cell , neural stem cell , chemistry , growth factor , immunology , in vitro , biochemistry , vesicle , receptor , membrane , synaptic vesicle , gene
Efficient 3D cell systems for neuronal induction are needed for future use in tissue regeneration. In this study, we have characterized the ability of neural stem/progenitor cells (NS/PC) to survive, proliferate, and differentiate in a collagen type I‐hyaluronan scaffold. Embryonic, postnatal, and adult NS/PC were seeded in the present 3D scaffold and cultured in medium containing epidermal growth factor and fibroblast growth factor‐2, a condition that stimulates NS/PC proliferation. Progenitor cells from the embryonic brain had the highest proliferation rate, and adult cells the lowest, indicating a difference in mitogenic responsiveness. NS/PC from postnatal stages down‐regulated nestin expression more rapidly than both embryonic and adult NS/PC, indicating a faster differentiation process. After 6 days of differentiation in the 3D scaffold, NS/PC from the postnatal brain had generated up to 70% neurons, compared with 14% in 2D. NS/PC from other ages gave rise to approximately the same proportion of neurons in 3D as in 2D (9–26% depending on the source for NS/PC). In the postnatal NS/PC cultures, the majority of βIII‐tubulin‐positive cells expressed glutamate, γ‐aminobutyric acid, and synapsin I after 11 days of differentiation, indicating differentiation to mature neurons. Here we report that postnatal NS/PC survive, proliferate, and efficiently form synapsin I‐positive neurons in a biocompatible hydrogel. © 2007 Wiley‐Liss, Inc.