Premium
Human embryonic stem cell‐derived neural precursors develop into neurons and integrate into the host brain
Author(s) -
Guillaume Daniel J.,
Johnson M. Austin,
Li XueJun,
Zhang SuChun
Publication year - 2006
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.21022
Subject(s) - olfactory bulb , neuroscience , embryonic stem cell , neural stem cell , human brain , biology , white matter , transplantation , stem cell , neural cell , microbiology and biotechnology , cell , central nervous system , medicine , biochemistry , radiology , magnetic resonance imaging , gene
Whether and how in‐vitro‐produced human neural precursors mature and integrate into the brain are crucial to the utility of human embryonic stem (hES) cells in treating neurological disorders. After transplantation into the ventricles of neonatal immune‐deficient mice, hES‐cell‐derived neural precursors stopped expressing the cell division marker Ki67, except in neurogenic areas, and differentiated into neurons and then glia in a temporal course intrinsic to that of human cells regardless of location. The human cells located in the gray matter became neurons in the olfactory bulb and striatum, whereas those in the white matter produced exclusively glia. Importantly, the grafted human cells formed synapses. Thus, the in‐vitro‐produced human neural precursors follow their intrinsic temporal program to produce neurons and glia and, in response to environmental signals, generate cells appropriate to their target regions and integrate into the brain. © 2006 Wiley‐Liss, Inc.