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Uncaria rhynchophylla, a Chinese medicinal herb, has potent antiaggregation effects on Alzheimer's β‐amyloid proteins
Author(s) -
Fujiwara Hironori,
Iwasaki Koh,
Furukawa Katsutoshi,
Seki Takashi,
He Mei,
Maruyama Masahiro,
Tomita Naoki,
Kudo Yukitsuka,
Higuchi Makoto,
Saido Takaomi C.,
Maeda Sumihiro,
Takashima Akihiko,
Hara Masahiko,
Ohizumi Yasushi,
Arai Hiroyuki
Publication year - 2006
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20891
Subject(s) - fibril , herb , chemistry , neuroprotection , traditional medicine , amyloid (mycology) , biochemistry , pharmacology , medicinal herbs , biology , medicine , inorganic chemistry
Because the deposition of β‐amyloid protein (Aβ) is a consistent pathological hallmark of Alzheimer's disease (AD) brains, inhibition of Aβ generation, prevention of Aβ fibril formation, or destabilization of preformed Aβ fibrils would be attractive therapeutic strategies for the treatment of AD. We examined the effects of several medicinal herbs used in traditional Chinese medical formulae on the formation and destabilization of Aβ fibrils by using the thioflavin T binding assay, atomic force microscopic imaging, and electrophoresis. Our study demonstrates that several of these herbs have potent inhibitory effects on fibril formation of both Aβ 1–40 and Aβ 1–42 in concentration‐dependent manners; in particular, Uncaria rhynchophylla inhibited Aβ aggregation most intensively. Significant destabilization of preformed Aβ 1–40 and Aβ 1–42 fibrils was also induced by Uncaria rhynchophylla as well as some other herb extracts. Three‐dimensional HPLC analysis indicated that the water extract of this herb contains several different chemical compounds, including oxindole and indol alkaloids, which have been regarded as neuroprotective. Our results suggest that Uncaria rhynchophylla has remarkably inhibitory effects on the regulation of Aβ fibrils, and we conclude that this medicinal herb could have the potency to be a novel therapeutic agent to prevent and/or cure AD. © 2006 Wiley‐Liss, Inc.