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Strain difference in susceptibility to experimental autoimmune encephalomyelitis between Albino Oxford and Dark Agouti rats correlates with disparity in production of IL‐17, but not nitric oxide
Author(s) -
Miljkovic Djordje,
StosicGrujicic Stanislava,
Markovic Milos,
Momcilovic Miljana,
Ramic Zorica,
MaksimovicIvanic Danijela,
Mijatovic Sanja,
Popadic Dusan,
Cvetkovic Ivana,
MostaricaStojkovic Marija
Publication year - 2006
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20883
Subject(s) - experimental autoimmune encephalomyelitis , nitric oxide , in vivo , encephalomyelitis , endocrinology , medicine , autoimmune disease , immunology , myelin basic protein , t cell , myelin , lymph , ex vivo , biology , immune system , multiple sclerosis , disease , central nervous system , pathology , microbiology and biotechnology
Albino Oxford (AO) rats, unlike Dark Agouti (DA) rats are resistant to the induction of experimental autoimmune encephalomyelitis (EAE). The reason for the resistance could be some restraining mechanism preventing auto‐aggressive cell activation at the level of draining lymph nodes (DLN) during the induction phase of the disease. Such a mechanism could be anti‐proliferative action of nitric oxide (NO), which has already been shown of importance for the resistance of several rat strains to the induction of the disease. Importantly, number of AO DLN cells (DLNC) is markedly lower and with lower proliferative response to myelin basic protein (MBP) ex vivo in comparison to DA DLNC in the inductive phase of EAE, thus implying that in AO rats DLNC do not proliferate as extensively as in DA rats. We show that AO rats do not produce larger quantities of NO than DA rats after immunization. Further, DLNC of immunized AO rats have significantly lower mRNA expression and synthesis of interferon (IFN)‐γ and interleukin (IL)‐17 compared to DLNC of DA rats. Collectively, these results suggest that there is a substantial difference between EAE‐resistant AO rats and EAE‐prone DA rats in the initiation of autoimmune response. This difference seems to be independent of anti‐proliferative actions of NO, but correlates with impaired IL‐17 production in AO rats. © 2006 Wiley‐Liss, Inc.