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Rat Schwann cells express M1–M4 muscarinic receptor subtypes
Author(s) -
Loreti Simona,
Vilaró M. Teresa,
Visentin S.,
Rees H.,
Levey Allan I.,
Tata Ada Maria
Publication year - 2006
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20874
Subject(s) - muscarinic acetylcholine receptor , muscarinic acetylcholine receptor m2 , muscarinic acetylcholine receptor m1 , muscarinic acetylcholine receptor m3 , microbiology and biotechnology , muscarinic acetylcholine receptor m5 , muscarinic acetylcholine receptor m4 , receptor , schwann cell , biology , stimulation , neuroscience , endocrinology , medicine , chemistry , biochemistry
The expression of different muscarinic receptor subtypes was analyzed in immature Schwann cells obtained from sciatic nerve of 2‐day neonatal rats. By using RT‐PCR analysis, we demonstrated the presence of M1, M2, M3, and M4 receptor subtypes in cultured Schwann cells, with M2 displaying the highest expression levels. Muscarinic subtypes were also quantified by immunoprecipitation and [ 3 H]QNB binding. With this approach, we found the levels of receptor expression to be M2 > M3 > M1. M4 is expressed at very low levels, and M5 receptor was not detectable. Moreover, we also demonstrated that stimulation of the receptors by muscarinic agonists activates previously described signal transduction pathways, leading to a decrease of cAMP and an increase of IP 3 levels not associated with an efficient intracellular Ca 2+ release. The presence and activity of particular muscarinic receptors in immature Schwann cells suggest that ACh may play an important role in Schwann cell development. © 2006 Wiley‐Liss, Inc.