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Neurogenic potential of human umbilical cord blood: Neural‐like stem cells depend on previous long‐term culture conditions
Author(s) -
Jurga M.,
Markiewicz I.,
Sarnowska A.,
Habich A.,
Kozlowska H.,
Lukomska B.,
Buzanska L.,
DomanskaJanik K.
Publication year - 2006
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20766
Subject(s) - umbilical cord , neural stem cell , stem cell , cord lining , placenta cord banking , neuroscience , term (time) , cord blood , medicine , biology , adult stem cell , microbiology and biotechnology , immunology , endothelial stem cell , fetus , physics , pregnancy , in vitro , genetics , quantum mechanics , placenta
In vitro studies conducted by our research group documented that neural progenitor cells can be selected from human umbilical cord blood (HUCB‐NPs). Due to further expansion of these cells we have established the first human umbilical cord blood‐derived neural‐like stem cell line (HUCB‐NSC) growing in serum‐free (SF) or low‐serum (LS) medium for over 3 years. The purpose of the study was to evaluate the neurogenic potential of HUCB‐NSCs cultured in SF and LS condition in different in vitro settings before transplantation. We have shown that the number of cells attaining neuronal features was significantly higher for cultures expanded in LS than in SF condition. Moreover, the presence of neuromorphogens, cultured rat astrocytes or hippocampal slices promoted further differentiation of HUCB‐NSCs into neural lineage much more effectively when the cells had derived from LS cultures. The highest response was observed in the case of co‐cultures with rat primary astrocytes as well as hippocampal organotypic slices. However, the LS cells co‐cultured with hippocampal slices expressed exclusively a set of early and late neuronal markers whereas no detection of cells with glial‐specific markers was possible. In conclusion, certain level of stem/progenitor cell commitment is important for optimal response of HUCB‐NSC on the neurogenic signals provided by surrounding environment in vitro. © 2006 Wiley‐Liss, Inc.