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Sensory neurite outgrowth on white matter astrocytes is influenced by intracellular and extracellular S100A4 protein
Author(s) -
Fang Z.,
Forslund N.,
Takenaga K.,
Lukanidin E.,
Kozlova E.N.
Publication year - 2006
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20743
Subject(s) - neurite , white matter , astrocyte , extracellular , regeneration (biology) , dorsal root ganglion , biology , microbiology and biotechnology , neuroscience , myelin , central nervous system , sensory system , in vitro , biochemistry , medicine , radiology , magnetic resonance imaging
Abstract The central nervous system (CNS) is considered a nonpermissive environment for axonal regeneration because of the presence of myelin and associated repulsive molecules. However, neural cells transplanted to the CNS preferably migrate and extend their fibers in white matter areas. We previously showed that white matter astrocytes in vivo express the calcium‐binding protein S100A4, which is strongly up‐regulated in areas of white matter degeneration. To investigate the role of white matter astrocytes and their specific protein S100A4 in axonal regeneration, we developed white matter astrocyte cultures with strong S100A4 expression and grew dissociated adult dorsal root ganglion (DRG) cells on top of astrocytes for 24 hr. By using small interfering S100A4 RNA, we were able to eliminate S100A4 expression and compare growth of DRG cell neurites on S100A4‐silenced and S100A4‐expressing astrocytes. In addition, we studied whether extracellular S100A4 has an effect on neurite growth from adult DRG cells cultured on S100A4‐expressing white matter astrocytes. Our data show that white matter astrocytes are permissive for neurite growth, although high levels of S100A4 in white matter astrocytes have a negative effect on this growth. Extracellular application of S100A4 induced extensive growth of DRG cell neurites on white matter astrocytes. These findings suggest that white matter astrocytes are able to support axonal regeneration and, furthermore, that administration of extracellular S100A4 provides strong additional support for axonal regeneration. © 2006 Wiley‐Liss, Inc.