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Localization of neuronal calcium sensor‐1 at the adult and developing rat neuromuscular junction
Author(s) -
Garcia Neus,
Lanuza Maria A.,
Besalduch Nuria,
Santafe Manel M.,
Jeromin Andreas,
Tomas Josep
Publication year - 2005
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20593
Subject(s) - synaptogenesis , neuromuscular junction , postsynaptic potential , axon , biology , neuroscience , neurotransmission , axon terminal , synapse , microbiology and biotechnology , confocal microscopy , calcium binding protein , neuromuscular transmission , calcium , anatomy , medicine , endocrinology , receptor , biochemistry
Neuronal calcium sensor (NCS‐1; frequenin) is a calcium‐binding protein involved in the regulation of neurotransmission in the central and peripheral nervous systems from insects to vertebrates. This study reports the localization of NCS‐1 immunoreactivity, by Western blotting and immunohistochemistry, at the adult and developing postnatal rat neuromuscular junction. Our confocal immunofluorescence results on the whole‐mount muscle and on semithin cross‐sections are indicative of the localization of NCS‐1 to motor axon terminals. There is no evidence of immunoreactivity in the postsynaptic side of the neuromuscular junctions or teloglial Schwann cells. These results suggest that NCS‐1 is involved in the formation and function of presynaptic nerve terminal part of the neuromuscular junction during synaptogenesis and in adult mammals. © 2005 Wiley‐Liss, Inc.