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Glutamate activation of Oct‐2 in cultured chick Bergmann glia cells: Involvement of NFκB
Author(s) -
Méndez J. Alfredo,
LópezBayghen Esther,
Ortega Arturo
Publication year - 2005
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20519
Subject(s) - ampa receptor , microbiology and biotechnology , metabotropic glutamate receptor 6 , glutamate receptor , creb , biology , activator (genetics) , metabotropic glutamate receptor 1 , metabotropic glutamate receptor 7 , signal transduction , kainate receptor , metabotropic glutamate receptor , metabotropic glutamate receptor 8 , metabotropic receptor , receptor , transcription factor , biochemistry , gene
Glutamate, the major excitatory neurotransmitter in the central nervous system, is critically involved in gene expression regulation at the transcriptional and translational levels. Its activity through ionotropic as well as metabotropic receptors modifies the protein repertoire in neurons and glial cells. In avian cerebellar Bergmann glia cells, glutamate receptors trigger a diverse array of signaling cascades that include activity‐dependent transcription factors such as the activator protein‐1, the cAMP response‐element binding protein, and Oct‐2. We analyze the upstream regulatory elements involved in Oct‐2 activation. Our results demonstrate that Ca 2+ influx, protein kinase C, phosphatidylinositol‐3 kinase, Src, and nuclear factor (NF)κB are involved in this signaling pathway. Our findings link α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate (AMPA) receptor activation to a negative phase of chkbp gene regulation, controlled by NFκB. © 2005 Wiley‐Liss, Inc.