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Synaptosomal dopamine uptake in rat striatum following controlled cortical impact
Author(s) -
Wilson Margaret S.,
Chen Xiangbai,
Ma Xiecheng,
Ren Dianxu,
Wagner Amy K.,
Reynolds Ian J.,
Dixon C. Edward
Publication year - 2005
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20419
Subject(s) - striatum , dopamine , neuroscience , chemistry , cortical neurons , psychology
Functional deficits following traumatic brain injury (TBI) are associated with alterations in markers of dopaminergic neurotransmission. To assess the effects of TBI on the expression and functional integrity of dopamine transporters, we measured transporter protein levels and investigated synaptosomal dopamine uptake in the rat striatum. Two or four weeks after lateral controlled cortical impact or sham injury, Western blotting revealed a decrease in transporter protein in the ipsilateral striatum of injured rats relative to shams ( P < 0.05). However, no significant difference in synaptosomal uptake (K m , V max ) was found between injured and sham‐injured animals. Our data suggest that striatal dopamine transporters are capable of normal function at 2 weeks and 4 weeks after injury. However, it is unclear whether neurons in the injured striatum can properly regulate the activity of dopamine transporters in vivo. © 2005 Wiley‐Liss, Inc.