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Estrogen promotes differentiation and survival of dopaminergic neurons derived from human neural stem cells
Author(s) -
Kishi Yo,
Takahashi Jun,
Koyanagi Masaomi,
Morizane Asuka,
Okamoto Yo,
Horiguchi Satoshi,
Tashiro Kei,
Honjo Tasuku,
Fujii Shingo,
Hashimoto Nobuo
Publication year - 2004
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20362
Subject(s) - dopaminergic , tyrosine hydroxylase , neural stem cell , estrogen receptor , estrogen , transplantation , biology , neuron , stem cell , cellular differentiation , estrogen receptor beta , neuroscience , endocrinology , dopamine , medicine , microbiology and biotechnology , cancer , biochemistry , genetics , breast cancer , gene
To investigate the effect of estrogen on neuronal differentiation, especially on dopaminergic (DA) neurons, human neural stem cells (NSCs) were differentiated in the presence of 17β‐estradiol. NSCs gave rise to tyrosine hydroxylase (TH)‐positive neurons in vitro, the proportion of which was increased by 17β‐estradiol. Increase in TH‐positive neurons was abrogated by an estrogen receptor (ER) antagonist, ICI182780, suggesting ERs play a role in differentiation of DA neurons. The observation that ERs were expressed in both proliferating NSCs and postmitotic DA neurons suggested that increase in TH‐positive neurons was due to induction and support of DA neurons. 17β‐Estradiol also increased the number of DA neurons derived from human NSCs in vivo when the cells were grafted into mouse brains. These results support a possible role for estrogen in the transplantation of NSCs for Parkinson's disease. © 2004 Wiley‐Liss, Inc.