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Differential gene expression in neural stem cells and oligodendrocyte precursor cells: A cDNA microarray analysis
Author(s) -
Hu JianGuo,
Fu SaiLi,
Zhang KaiHua,
Li Ying,
Yin Lan,
Lu PeiHua,
Xu XiaoMing
Publication year - 2004
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20317
Subject(s) - neural stem cell , biology , oligodendrocyte , forebrain , precursor cell , complementary dna , stem cell , embryonic stem cell , microbiology and biotechnology , microarray , microarray analysis techniques , gene , cellular differentiation , gene expression , in vitro , myelin , neuroscience , genetics , central nervous system
The use of neural stem cells (NSCs) or their progeny oligodendrocyte precursor cells (OPCs) represents a promising repair strategy for many neurological disorders. However, the molecular events and biological features during the transition from NSCs to OPCs remain unclear. In the present study, we isolated NSCs from the embryonic rat forebrain and induced them into OPCs by using B104 conditioned medium (B104CM) in vitro. We then employed cDNA array technology to compare changes in gene expression between the two cell populations. Among 1,176 genes examined, 40 were differentially expressed, and some of them may be involved in OPC differentiation from NSCs. Our findings thus provide new insights into the molecular basis of differentiation of OPCs from NSCs. © 2004 Wiley‐Liss, Inc.