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Expression and location of mRNAs encoding multiple forms of secretory phospholipase A 2 in the rat retina
Author(s) -
Kolko Miriam,
Christoffersen Nanna R.,
Barreiro Sebastian G.,
Bazan Nicolas G.
Publication year - 2004
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20187
Subject(s) - biology , retina , retinal , in situ hybridization , complementary dna , messenger rna , intracellular , phospholipase , microbiology and biotechnology , receptor , cloning (programming) , gene , rna , enzyme , biochemistry , neuroscience , computer science , programming language
Abstract Low‐molecular‐weight secretory phospholipases A 2 (sPLA 2 s) are a subgroup of PLA 2 s, which are secreted, bind to receptors, and may act as intercellular signaling modulators. At least 10 different groups have been characterized in mammals, and there is expanding evidence of the significance of sPLA 2 s in neuronal signaling and survival [Kolko et al. (1996) J. Biol. Chem. 271: 32722–32728]. To date, no retinal sPLA 2 s have been cloned or characterized. We evaluated the existence and abundance of sPLA 2 subtypes in rat retina and explored their possible involvement in light‐induced retinal damage. We designed primers to identify the sPLA 2 s in rat retina, based on known sequences of sPLA 2 ‐specific mRNAs in other tissues. RNA was isolated from rat retina, and cDNA was produced and used for PCR cloning to identify the novel subtypes of sPLA 2 . Our study revealed the presence of mRNAs encoding sPLA 2 ‐IB, ‐X, ‐V, ‐IIE, ‐IIA, and ‐IIF in the retina, and quantification by real‐time PCR revealed different abundances of the sPLA 2 s. We showed a time‐dependent gene induction of sPLA 2 ‐X, ‐IB, and ‐V in light‐induced retinal damage. We further explored the location of sPLA 2 ‐IB by in situ hybridization and immunohistochemistry. This study is the first to reveal the presence, abundance, and induction of mRNAs encoding sPLA 2 s in rat retina. We suggest that these enzymes are themselves intercellular signaling modulators of retinal cell function and perhaps also of retinal degeneration. © 2004 Wiley‐Liss, Inc.