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Estradiol alters transcription factor gene expression in primate prefrontal cortex
Author(s) -
Wang J.,
Cheng C.M.,
Zhou J.,
Smith A.,
Weickert C. Shan,
Perlman W.R.,
Becker K.G.,
Powell D.,
Bondy C.A.
Publication year - 2004
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20076
Subject(s) - estrogen , ovariectomized rat , dorsolateral prefrontal cortex , prefrontal cortex , in situ hybridization , biology , transcription factor , primate , estrogen receptor , gene expression , neuroscience , medicine , endocrinology , gene , genetics , cognition , cancer , breast cancer
Estrogen protects neurons from a variety of experimental insults in vitro, and is thought to protect from acute and chronic neurodegenerative processes in vivo. Estrogen also enhances higher‐level cognitive functions that are centered in the dorsolateral prefrontal cortex (DLPFC) in human and non‐human primates. To investigate genomic mechanisms involved in estrogenic effects on the primate brain in vivo, we compared transcription factor mRNA and protein expression in the DLPFC of ovariectomized rhesus monkeys treated with either vehicle or estradiol (E2). c‐FOS, E2F1, and general transcription factor IIB (TFIIB) mRNA and protein expression were altered significantly by short‐term E2 treatment, as shown by DNA array, in situ hybridization, and immunohistochemical and immunoblot evaluations. C‐FOS expression was increased significantly whereas E2F1 and TFIIB levels were decreased in the DLPFC of E2‐treated animals. These transcription factors were concentrated in cortical pyramids, as were estrogen receptors α and β. These data indicate that estrogen may have direct as well as indirect effects on neuronal gene expression in the primate prefrontal cortex. © 2004 Wiley‐Liss, Inc.