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Stromal cell‐derived factor 1‐mediated CXCR4 signaling in rat and human cortical neural progenitor cells
Author(s) -
Peng Hui,
Huang Yunlong,
Rose Jeremy,
Erichsen David,
Herek Shelley,
Fujii Nobutaka,
Tamamura Hirokazu,
Zheng Jialin
Publication year - 2004
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.20045
Subject(s) - progenitor cell , stromal cell , neural stem cell , neuroscience , microbiology and biotechnology , cxcr4 , stromal cell derived factor 1 , biology , stem cell , cancer research , receptor , biochemistry , chemokine
Stromal cell‐derived factor 1 (SDF‐1) and the chemokine receptor CXCR4 are highly expressed in the nervous system. Knockout studies have suggested that both SDF‐1 and CXCR4 play essential roles in cerebellar, hippocampal, and neocortical neural cell migration during embryogenesis. To extend these observations, CXCR4 signaling events in rat and human neural progenitor cells (NPCs) were examined. Our results show that CXCR4 is expressed in abundance on rat and human NPCs. Moreover, SDF‐1α induced increased NPCs levels of inositol 1,4,5‐triphosphate, extracellular signal‐regulated kinases 1/2, Akt, c‐Jun N‐terminal kinase, and intracellular calcium whereas it diminished cyclic adenosine monophosphate. Finally, SDF‐1α can induce human NPC chemotaxis in vitro, suggesting that CXCR4 plays a functional role in NPC migration. Both T140, a CXCR4 antagonist, and pertussis toxin (PTX), an inactivator of G protein‐coupled receptors, abrogated these events. Ultimately, this study suggested that SDF‐1α can influence NPC function through CXCR4 and that CXCR4 is functional on NPC. © 2004 Wiley‐Liss, Inc.