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Role of rRAB22b, an oligodendrocyte protein, in regulation of transport of vesicles from trans Golgi to endocytic compartments
Author(s) -
RodriguezGabin A.G.,
Cammer M.,
Almazan G.,
Charron M.,
Larocca J.N.
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.1253
Subject(s) - golgi apparatus , endocytic cycle , microbiology and biotechnology , endosome , rab , vesicle , biology , vesicular transport protein , transport protein , oligodendrocyte , copi , organelle , secretory pathway , brefeldin a , intracellular , myelin , endocytosis , endoplasmic reticulum , biochemistry , gtpase , membrane , cell , neuroscience , central nervous system
Intracellular membrane trafficking plays an essential role in the biogenesis and maintenance of myelin. Members of the Rab protein family are important components of the systems that regulate intracellular vesicle transport. We examine the function of rRab22b, a novel rat Rab protein cloned from an oligodendrocyte cDNA library, by visualizing and identifying in living Hela cells the organelles that contain rRab22b. Our results show that rRab22b is present in the trans Golgi/TGN and endocytic compartments. Trafficking of membranes from trans Golgi to endocytic compartments takes place via small tubulo vesicular organelles containing rRab22b. The formation of vesicles in the trans Golgi also appears to be regulated by rRab22b. Additionally, our results suggest that rRab22b controls the transport of vesicles from the trans Golgi to endocytic compartments that localize in oligodendrocyte processes. That rRab22b is involved in the transport of certain proteins from trans Golgi to myelin is suggested by the evidence that certain proteins being targeted to the plasma membrane are first transported from trans Golgi to endocytic compartments. J Neurosci. Res. 66:1149–1160, 2001. © 2001 Wiley‐Liss, Inc.