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Axon‐Schwann cell interactions regulate the expression of fibroblast growth factor‐5 (FGF‐5)
Author(s) -
Scarlato Marina,
Xu Theodore,
Bannerman Peter,
Beesley Jacqueline,
Reddy Usha R.,
Rostami Abdolmohammad,
Scherer Steven S.,
Pleasure David
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.1193
Subject(s) - wallerian degeneration , schwann cell , fibroblast growth factor , axon , blot , microbiology and biotechnology , sciatic nerve , biology , in situ hybridization , axotomy , fibroblast , northern blot , messenger rna , neuroscience , cell culture , anatomy , gene , genetics , receptor , regeneration (biology)
We screened for genes whose expression is significantly up‐ or downregulated during Wallerian degeneration in adult rat sciatic nerve with cDNA arrays. Fibroblast growth factor‐5 (FGF‐5) mRNA seemed to be induced. This was confirmed by northern blotting and in situ hybridization, as well as Western blotting for FGF‐5 in axotomized nerve. Axon‐Schwann cell interactions decreased the steady‐state level of FGF‐5 mRNA in regenerating sciatic nerves, and forskolin diminished its expression in cultured Schwann cells. We conclude that denervated Schwann cells synthesize FGF‐5, which is a secreted, neuronotrophic member of the FGF family. J. Neurosci. Res. 66:16–22, 2001. © 2001 Wiley‐Liss, Inc.