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Increase of oligodendrocyte progenitor cells after spinal cord injury
Author(s) -
Ishii Ken,
Toda Masahiro,
Nakai Yoko,
Asou Hiroaki,
Watanabe Masahiko,
Nakamura Masaya,
Yato Yoshiyuki,
Fujimura Yoshikazu,
Kawakami Yutaka,
Toyama Yoshiaki,
Uyemura Keiichi
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.1180
Subject(s) - galactocerebroside , remyelination , lesion , oligodendrocyte , spinal cord , progenitor cell , spinal cord injury , immunohistochemistry , pathology , myelin , laminectomy , progenitor , medicine , anatomy , neuroscience , biology , stem cell , central nervous system , microbiology and biotechnology
The reaction of oligodendrocyte progenitor cells (OPCs) after spinal cord injury (SCI) is poorly understood. In this study, we examined oligodendroglial reactions after contusion SCI in adult rats by immunohistochemistry. OPCs were identified by staining with monoclonal antibodies (mAbs) A2B5 and O4. Each of the A2B5‐, O4‐positive OPCs and galactocerebroside‐positive oligodendrocytes dramatically increased in the lesion of the dorsal posterior funiculus. Bromodeoxyuridine (BrdU) incorporation studies showed that most O4‐positive cells in the lesion were labeled with BrdU, suggesting that these OPCs were proliferative. In contrast, the expression of myelin basic protein was decreased in the lesion compared with controls that received laminectomy only. From the injured cord, OPCs were isolated by immunopanning with mAb A2B5. We observed an increased number of OPCs from the injured spinal cords compared with those isolated from controls and unoperated animals. After several days in culture, the OPCs from the lesion expressed galactocerebroside. These results suggest that OPCs are induced and can differentiate following SCI in the adult rat. J. Neurosci. Res. 65:500–507, 2001. © 2001 Wiley‐Liss, Inc.