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Neurofilaments of Klotho , the mutant mouse prematurely displaying symptoms resembling human aging
Author(s) -
Uchida Atsuko,
Komiya Yoshiaki,
Tashiro Tomoko,
Yorifuji Hiroshi,
Kishimoto Takeo,
Nabeshima Yohichi,
Hisanaga Shinichi
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.1087
Subject(s) - neurofilament , mutant , neuroscience , microbiology and biotechnology , biology , genetics , immunology , gene , immunohistochemistry
We reported previously that neurofilaments (NFs) of aged rats were highly packed in the axon and contained a smaller amount of NF‐M as compared with those of young rats (Uchida et al. [1999] J. Neurosci. Res. 58:337–348). We studied NFs of the mutant mouse, named Klotho , which displays prematurely symptoms resembling human aging. The transport of axonal cytoskeletal proteins, including NFs, tubulin and actin, was decreased at the leading portion of the peak of transported proteins in Klotho during the process of premature aging. The nearest neighbor inter‐NF distance in Klotho axons (35–39 nm) was shorter than that of the wild‐type mouse (48–49 nm), indicating the packing of NFs in Klotho . The ratio of NF‐M to NF‐L was slightly decreased in cytoskeletons from the spinal cords of Klotho . These changes are similar, though not identical, to those observed in aged rats, and are the first evidence of age‐related changes in the neurons of Klotho . J. Neurosci. Res. 64:364–370, 2001. © 2001 Wiley‐Liss, Inc.