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Relationships between cholinergic phenotype and acetyl‐CoA level in hybrid murine neuroblastoma cells of septal origin
Author(s) -
Bielarczyk Hanna,
Tomaszewicz Maria,
Madziar Beata,
Ćwikowska Justyna,
Pawełczyk Tadeusz,
Szutowicz Andrzej
Publication year - 2003
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10711
Subject(s) - choline acetyltransferase , cholinergic , acetylcholine , transfection , cholinergic neuron , choline , neuroblastoma , retinoic acid , acetyltransferase , cell culture , biology , biochemistry , chemistry , microbiology and biotechnology , endocrinology , acetylation , gene , genetics
High susceptibility of cholinergic neurons to neurotoxic signals may result from their utilization of acetyl‐CoA for both energy production and acetylcholine synthesis. SN56 cholinergic cells were transfected stably with cDNA for choline acetyltransferase. Transfected cells (SN56ChAT2) expressed choline acetyltransferase activity and acetylcholine content, 17 times and 2 times higher, respectively, than did nontransfected cells. Transfection did not change pyruvate dehydrogenase but decreased the acetyl‐CoA level by 62%. Differentiation by cAMP and retinoic acid caused an increase of choline acetyltransferase activity and decrease of acetyl‐CoA levels in both cell lines. Negative correlation was found between choline acetyltransferase activity and acetyl‐CoA level in these cells. SN56ChAT2 cells were more susceptible to excess NO than were native SN56 cells, as evidenced by the thiazolyl blue reduction assay. Thus, the sensitivity of cholinergic neurons to pathologic conditions may depend on the cholinergic phenotype‐dependent availability of acetyl‐CoA. © 2003 Wiley‐Liss, Inc.