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Increased turnover of Na‐K ATPase molecules in rat brain after rapid eye movement sleep deprivation
Author(s) -
Majumdar Sudipta,
Faisal Mohd,
Madan Vibha,
Mallick Birendra N.
Publication year - 2003
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10710
Subject(s) - rapid eye movement sleep , atpase , sleep deprivation , enzyme , western blot , sleep (system call) , chemistry , medicine , enzyme assay , endocrinology , eye movement , biochemistry , neuroscience , biology , circadian rhythm , gene , computer science , operating system
It has been shown that rapid eye movement (REM) sleep deprivation increases Na‐K ATPase activity. Based on kinetic study, it was proposed that increased activity was due to enhanced turnover of enzyme molecules. To test this, anti‐α1 Na‐K ATPase monoclonal antibody (mAb 9A7) was used to label Na‐K ATPase molecules. These labeled enzymes were quantified on neuronal membrane by two methods: histochemically on neurons in tissue sections from different brain areas, and by Western blot analysis in control and REM sleep‐deprived rat brains. The specific enzyme activity was also estimated and found to be increased, as in previous studies. The results confirmed our hypothesis that after REM sleep deprivation, increased Na‐K ATPase activity was at least partly due to increased turnover of Na‐K ATPase molecules in the rat brain. © 2003 Wiley‐Liss, Inc.