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Olanzapine protects PC12 cells from oxidative stress induced by hydrogen peroxide
Author(s) -
Wei Zelan,
Bai Ou,
Richardson J. Steven,
Mousseau Darrell D.,
Li XinMin
Publication year - 2003
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10668
Subject(s) - olanzapine , superoxide dismutase , oxidative stress , pharmacology , hydrogen peroxide , chemistry , catalase , medicine , endocrinology , schizophrenia (object oriented programming) , biochemistry , psychiatry
Neuroanatomical studies suggest that neuronal atrophy and destruction occur over the course of many years in neurodegenerative conditions such as schizophrenia and Alzheimer's disease. In schizophrenia, early intervention with atypical neuroleptics such as olanzapine has been shown to prevent development of some of the more serious and debilitating symptoms in many patients. The mechanisms whereby olanzapine slows or prevents symptom progression in schizophrenia remain unclear. A previous study found that olanzapine increased mRNA for the copper/zinc isoform of the superoxide dismutase enzyme (SOD‐1). We investigated the effects of olanzapine in PC12 cells exposed to hydrogen peroxide. We measured cell viability, observed evidence of necrosis and apoptosis, checked the SOD‐1 mRNA by Northern blot analyses, and determined SOD‐1 enzyme activity. We found that: 1) the decrease in cell viability induced by hydrogen peroxide was attenuated in PC12 cells pretreated with olanzapine; 2) olanzapine increased SOD enzyme activity in PC12 cells; 3) inhibiting SOD activity with diethyldithiocarbamic acid prevented the cytoprotective actions of olanzapine; and 4) the decrease in SOD‐1 mRNA level induced by hydrogen peroxide was blocked by pretreatment with olanzapine. These data indicate that the neuroprotective action of olanzapine includes the upregulation of SOD. © 2003 Wiley‐Liss, Inc.