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Vasoactive intestinal peptide and nitric oxide promote survival of adult rat myenteric neurons in culture
Author(s) -
Sandgren Katarina,
Lin Zhong,
Svenningsen Åsa Fex,
Ekblad Eva
Publication year - 2003
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10612
Subject(s) - vasoactive intestinal peptide , nitric oxide , enteric nervous system , nitric oxide synthase , motility , biology , myenteric plexus , antiserum , medicine , endocrinology , neuropeptide , immunohistochemistry , microbiology and biotechnology , immunology , biochemistry , antibody , receptor
Several motility disorders originate in the enteric nervous system (ENS). Our knowledge of factors governing survival of the ENS is poor. Changes in the expression of vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in enteric neurons occur after neuronal injury and in intestinal adaptation. The aim of this study was to evaluate whether VIP and nitric oxide (NO) influence survival of cultured, dissociated myenteric neurons. Neuronal survival was evaluated after 0, 4, and 8 days in culture. Influence of VIP and NO on neuronal survival was examined after culturing in the presence of VIP, NO donor, VIP antiserum, or NOS inhibitor. A marked loss of neurons was noted during culturing. VIP and NO significantly promoted neuronal survival. Corroborating this was the finding of an enhanced neuronal cell loss when cultures were grown in the presence of VIP antiserum or NOS inhibitor. © 2003 Wiley‐Liss, Inc.

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