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Implication of cyclooxygenase‐2 on enhanced proliferation of neural progenitor cells in the adult mouse hippocampus after ischemia
Author(s) -
Sasaki Tsutomu,
Kitagawa Kazuo,
Sugiura Shiro,
OmuraMatsuoka Emi,
Tanaka Shigeru,
Yagita Yoshiki,
Okano Hideyuki,
Matsumoto Masayasu,
Hori Masatsugu
Publication year - 2003
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10595
Subject(s) - dentate gyrus , subgranular zone , neurogenesis , neural stem cell , progenitor cell , hippocampus , neun , neuroscience , hippocampal formation , bromodeoxyuridine , ischemia , biology , medicine , stem cell , microbiology and biotechnology , immunology , subventricular zone , immunohistochemistry
Abstract Global ischemia promotes neurogenesis in the dentate gyrus of the adult mouse hippocampus. Cyclooxygenase (COX)‐2, the principal isoenzyme in the brain, modulates inflammation, glutamate‐mediated cytotoxicity, and synaptic plasticity. We demonstrated that delayed treatment with different classes of COX inhibitor significantly blunted enhancement of dentate gyrus proliferation of neural progenitor cells after ischemia. COX‐2 immunoreactivity was observed in both neurons and astrocytes in the dentate gyrus, but not in neural progenitor cells in the subgranular zone. Moreover, in the postischemic dentate gyrus of heterozygous and homozygous COX‐2 knockout mice, proliferating bromodeoxyuridine‐positive cells were significantly fewer than in wild‐type littermates. These results demonstrate that COX‐2 is an important modulator in enhancement of proliferation of neural progenitor cells after ischemia. © 2003 Wiley‐Liss, Inc.