Premium
Adenoviral gene transfer of GDNF, BDNF and TGFβ2, but not CNTF, cardiotrophin‐1 or IGF1, protects injured adult motoneurons after facial nerve avulsion
Author(s) -
Sakamoto Tsuyoshi,
Kawazoe Yoko,
Shen JinSong,
Takeda Yasuo,
Arakawa Yoshihiro,
Ogawa Junko,
Oyanagi Kiyomitsu,
Ohashi Toya,
Watanabe Kazutada,
Inoue Kiyoharu,
Eto Yoshikatsu,
Watabe Kazuhiko
Publication year - 2003
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10558
Subject(s) - ciliary neurotrophic factor , glial cell line derived neurotrophic factor , choline acetyltransferase , neurotrophic factors , nerve growth factor , endocrinology , medicine , transforming growth factor , biology , neuroscience , cholinergic , receptor
We examined neuroprotective effects of recombinant adenoviral vectors encoding glial cell line‐derived neurotrophic factor (GDNF), brain‐derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), cardiotrophin‐1 (CT1), insulin‐like growth factor‐1 (IGF1), and transforming growth factor‐β2 (TGFβ2) on lesioned adult rat facial motoneurons. The right facial nerves of adult Fischer 344 male rats were avulsed and removed from the stylomastoid foramen, and adenoviral vectors were injected into the facial canal. Animals avulsed and treated with adenovirus encoding GDNF, BDNF, CNTF, CT1, IGF1 and TGFβ2 showed intense immunolabeling for these factors in lesioned facial motoneurons, respectively, indicating adenoviral induction of the neurotrophic factors in these neurons. The treatment with adenovirus encoding GDNF, BDNF, or TGFβ2 after avulsion significantly prevented the loss of lesioned facial motoneurons, improved choline acetyltransferase immunoreactivity and prevented the induction of nitric oxide synthase activity in these neurons. The treatment with adenovirus encoding CNTF, CT1 or IGF1, however, failed to protect these neurons after avulsion. These results indicate that the gene transfer of GDNF and BDNF and TGFβ2 but not CNTF, CT1 or IGF1 may prevent the degeneration of motoneurons in adult humans with motoneuron injury and motor neuron diseases. © 2003 Wiley‐Liss, Inc.