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Differential expression of L‐ and S‐MAG upon cAMP stimulated differentiation in oligodendroglial cells
Author(s) -
Erb M.,
Steck A.J.,
Nave K.A.,
SchaerenWiemers N.
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10497
Subject(s) - biology , alternative splicing , gene isoform , cellular differentiation , microbiology and biotechnology , rna splicing , exon , myelin associated glycoprotein , gene expression , gene , myelin , rna , genetics , central nervous system , neuroscience
Myelin‐associated glycoprotein (MAG), an immunoglobulin‐like cell signaling protein involved in axon‐glial interactions, displays two intracellular C‐termini as a result of alternative mRNA splicing. During brain development, the two MAG mRNAs that encode L‐MAG and S‐MAG differ in their relative abundance. We have investigated the differential expression of L‐ and S‐MAG upon cAMP treatment in the oligodendroglial cell line Oli‐neu, a cell line able to differentiate in vitro. We have engineered GFP and VSVG fusions by small insertions into the alternatively spliced exons of the cloned MAG gene and reintroduced them into Oli‐neu cells. The individually tagged MAG isoforms were expressed under the control of the MAG promoter and regulatory region. In this system, L‐MAG was the predominant isoform before the stimulation of cells with cAMP, whereas upon cAMP treatment the S‐MAG isoform was predominantly expressed in cells with a high degree of morphological differentiation. We suggest that the regulation of the MAG alternative splicing and the morphological differentiation in oligodendrocytes are controlled both by the same cAMP‐responsive differentiation step. © 2002 Wiley‐Liss, Inc.