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Mature myelin basic protein‐expressing oligodendrocytes are insensitive to kainate toxicity
Author(s) -
Rosenberg Paul A.,
Dai Weimin,
Gan Xiao Dong,
Ali Sanjida,
Fu Jennifer,
Back Stephen A.,
Sanchez Russell M.,
Segal Michael M.,
Follett Pamela L.,
Jensen Frances E.,
Volpe Joseph J.
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10472
Subject(s) - kainate receptor , excitotoxicity , oligodendrocyte , glutamate receptor , biology , myelin basic protein , microbiology and biotechnology , agonist , myelin , neuroscience , receptor , biochemistry , ampa receptor , central nervous system
We examined the vulnerability to excitotoxicity of rat oligodendrocytes in dissociated cell culture at different developmental stages. Mature oligodendrocytes that express myelin basic protein were resistant to excitotoxic injury produced by kainate, whereas earlier stages in the oligodendrocyte lineage were vulnerable to this insult. To test the hypothesis that the sensitivity of immature oligodendrocytes and the resistance of mature oligodendrocytes to kainate toxicity were due to differences in membrane responsiveness to kainate, we used whole‐cell patch‐clamp recording. Oligodendrocyte precursors in cultures vulnerable to kainate toxicity responded to 500 μM kainate with large inward currents, whereas mature myelin basic protein‐expressing oligodendrocytes in cultures resistant to kainate toxicity showed no clear response to application of this agonist. We assayed expression of glutamate receptor subunits (GluR) ‐2, ‐4, ‐6, ‐7, and KA2 using immunoblot analysis and found that expression of all of these glutamate receptors was significantly down‐regulated in mature oligodendrocytes. These results suggest a striking developmental regulation of glutamate receptors in oligodendrocytes and suggest that the vulnerability of oligodendrocytes to non‐ N‐methyl‐D‐aspartate receptor‐mediated excitotoxicity might be much greater in developing oligodendrocytes than after the completion of myelination. © 2002 Wiley‐Liss, Inc.