Premium
Modification of representational difference analysis applied to the isolation of forskolin‐regulated genes from Schwann cells
Author(s) -
Bermingham John R.,
Shumas Sue,
Whisenhunt Tom,
Rosenfeld Michael G.,
Scherer Steven S.
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.1046
Subject(s) - forskolin , schwann cell , biology , microbiology and biotechnology , adenylyl cyclase , gene , activator (genetics) , mapk/erk pathway , signal transduction , cell culture , genetics
Many aspects of the response of Schwann cells to axonal cues can be induced in vitro by the adenylyl cyclase activator forskolin, yet the role of cAMP signaling in regulating Schwann cell differentiation remains unclear. To define better the relationship between cAMP signaling and Schwann cell differentiation, we used a modification of cDNA representational difference analysis (RDA) that permits the analysis of small amounts of mRNA and identified additional genes that are differentially expressed by forskolin‐treated and untreated Schwann cells. The genes that we have identified, including MKP3, a regulator of ERK signaling, and the sphingosine‐1‐phosphate receptor edg3/lp B3 , may play important roles in mediating Schwann cell differentiation. J. Neurosci. Res. 63:516–524, 2001. © 2001 Wiley‐Liss, Inc.