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Neural cell adhesion molecule‐mediated neurite outgrowth is repressed by overexpression of HES‐1
Author(s) -
Jessen Ulla,
Novitskaya Vera,
Walmod Peter S.,
Berezin Vladimir,
Bock Elisabeth
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10433
Subject(s) - neurite , neural cell adhesion molecule , microbiology and biotechnology , cell adhesion , cell adhesion molecule , transfection , intracellular , signal transduction , chemistry , biology , cell culture , cell , in vitro , biochemistry , genetics
The neural cell adhesion molecule (NCAM) stimulates neurite outgrowth by activating intracellular signaling cascades. We investigated the role of the transcriptional repressor HES‐1 in NCAM‐dependent neurite outgrowth by estimating neurite extension from PC12‐E2 cells grown in coculture with NCAM‐negative or NCAM‐positive fibroblasts. PC12‐E2 cells were transiently transfected with an expression plasmid encoding HES‐1. We found that expression of HES‐1 inhibited NCAM‐dependent neurite outgrowth. Treatment with arachidonic acid (an important messenger in NCAM‐dependent signaling) restored NCAM‐induced neurite outgrowth inhibited by HES‐1. These results suggest that HES‐1 is a regulator of intracellular signal transduction stimulated by cell adhesion molecules involved in neurite outgrowth. © 2002 Wiley‐Liss, Inc.