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Protein tyrosine phosphatase σ‐deficient mice show aberrant cytoarchitecture and structural abnormalities in the central nervous system
Author(s) -
Meathrel Karen,
Adamek Tamara,
Batt Jane,
Rotin Daniela,
Doering Laurie C.
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10382
Subject(s) - biology , knockout mouse , glial fibrillary acidic protein , protein tyrosine phosphatase , central nervous system , microbiology and biotechnology , neuroscience , pathology , receptor , genetics , immunology , immunohistochemistry , phosphorylation , medicine
Abstract Protein tyrosine phosphatase σ (PTPσ) is a member of the LAR family of receptor tyrosine phosphatases and is highly expressed in the nervous system during development. PTPσ is homologous to the Drosophila DLAR, which plays a key role in the targeting of axonal growth cones in flies. We have previously inactivated the Ptprs gene in mice and demonstrated stunted growth, developmental delays, and neurological and neuroendocrine defects in the PTPσ null animals. Here, we mapped the expression of the lac‐Z reporter gene included in the knockout cassette and surveyed the development of the CNS in these mice after birth. The strongest expression of β‐galactosidase (PTPσ) was observed in the hippocampus, cerebral cortex, olfactory bulbs, and subependymal layer. Our analysis reveals hippocampal dysgenesis, reductions in the thickness of the corpus callosum and the cerebral cortex, and late expression of the growth‐associated protein 43 (GAP‐43) in the knockout animals. Architectural abnormalities in the brain and spinal cord were confirmed by immunoreactivity to neurofilament and glial fibrillary acidic protein (GFAP) antibodies. Several of these neural abnormalities were corrected with age, suggesting a delay in neurological development related to the knockout of the Ptprs gene. These data suggest that PTPσ is likely involved in neurogenesis, axonal growth, and axonal pathfinding in the maturation of the mammalian CNS. © 2002 Wiley‐Liss, Inc.

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