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Turnover of synaptic membranes: Age‐related changes and modulation by dietary restriction
Author(s) -
Ando Susumu,
Tanaka Yasukazu,
Toyoda nee Ono Yuriko,
Kon Kazuo,
Kawashima Seiichi
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10352
Subject(s) - synaptophysin , phosphatidylethanolamine , membrane , phosphatidylcholine , synaptic vesicle , synaptic membrane , chemistry , biochemistry , biology , microbiology and biotechnology , biophysics , phospholipid , vesicle , immunohistochemistry , immunology
We examined age‐related changes in the turnover rates of synaptic membrane components that might underlie the decrease in synaptic functions in senescence. Synaptic membrane constituents were labeled in vivo with deuterium and the disappearance of the deuterated molecules from synaptic membranes was measured by mass spectrometry. The turnover rates of phosphatidylcholine, phosphatidylethanolamine, cholesterol, and synaptophysin were all shown to slow down with aging. Dietary restriction, which is known to retard various aging processes, was found to decrease the turnover rates of membrane lipid species. Consequently, the fatty acid composition in phospholipids remained unchanged in the synaptic plasma membranes of food restricted mice. In contrast, the turnover rate of synaptophysin was accelerated under dietary restriction. This may mean that increased turnover enhances the removal of damaged proteins from membranes. © 2002 Wiley‐Liss, Inc.