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Apoptotic death following Fas activation in human oligodendrocyte hybrid cultures
Author(s) -
Li Weiping,
Maeda Yasuhiro,
Ming Xue,
Cook Stuart,
Chapin John,
Husar Walter,
Dowling Peter
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10285
Subject(s) - fas ligand , flow cytometry , apoptosis , oligodendrocyte , programmed cell death , biology , microbiology and biotechnology , fas receptor , cell culture , biochemistry , genetics , myelin , neuroscience , central nervous system
The purpose of this study was to determine how oligodendrocytes die following Fas receptor activation. An immortalized human oligodendrocyte hybrid line (MO3.13) was challenged with Fas ligand (FasL), and cell death was assessed by flow cytometry and DNA gel electrophoresis. Caspase activation was determined by either Western immunoblotting on cell extracts or by whole‐cell flow cytometry. FasL challenge clearly induced substantial apoptotic cell death in the oligodendrocyte hybrid cell line, as judged by flow cytometry and by the presence of prominent low molecular weight DNA banding patterns after gel electrophoresis. Western immunoblots showed marked increases in cleaved caspase‐1, 8, and 3, indicating that the extrinsic Fas death receptor‐induced pathway was activated. The intrinsic mitochondrial pathway was also activated, but only at a minimal level. These findings demonstrate that there are several independent molecular sites within the extrinsic caspase cascade in oligodendrocytes where inhibitory compounds may be capable of blocking cell death in vivo. Published 2002 Wiley‐Liss, Inc.