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Rev‐erbα gene expression in the mouse brain with special emphasis on its circadian profiles in the suprachiasmatic nucleus
Author(s) -
Onishi Hiroyasu,
Yamaguchi Shun,
Yagita Kazuhiro,
Ishida Yoshiki,
Dong Xin,
Kimura Hidehito,
Jing Zhang,
Ohara Hidefumi,
Okamura Hitoshi
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10226
Subject(s) - suprachiasmatic nucleus , circadian rhythm , biology , gene expression , in situ hybridization , nucleus , messenger rna , microbiology and biotechnology , circadian clock , transcription (linguistics) , clock , medicine , endocrinology , gene , neuroscience , genetics , linguistics , philosophy
Rev‐erbα is an orphan nuclear receptor that constitutively suppresses gene transcription. In the present study, the expression of Rev‐erbα was investigated in the mouse brain by in situ hybridization using antisense cRNA probe. Positive Rev‐erbα mRNA signals were detected widely in the brain with the highest expression in the suprachiasmatic nucleus (SCN). In the constant dark condition, the circadian expression profiles of Rev‐erbα m RNA in the SCN showed a peak at early daytime (CT4) and a trough at early night time (CT16). The environmental lighting condition (light–dark environmental condition and exposure in the subjective night) did not alter the expression profiles. These findings indicate that Rev‐erbα gene is a transcription factor intimately related to the circadian clock in the SCN. © 2002 Wiley‐Liss, Inc.