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Suppression of BDNF‐induced expression of neuropeptide Y (NPY) in cortical cultures by oxygen‐glucose deprivation: A model system to study ischemic mechanisms in the perinatal brain
Author(s) -
Barnea Ayalla,
Roberts Jodie
Publication year - 2002
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10191
Subject(s) - neuropeptide y receptor , endocrinology , medicine , immunostaining , oxygen , privation , biology , chemistry , neuropeptide , immunohistochemistry , receptor , organic chemistry , sleep deprivation , circadian rhythm
The aim of this study was to establish a culture system that can serve as a model to study hypoxic‐ischemic mechanisms regulating the functional expression of NPY neurons in the perinatal brain. Using an aggregate culture system derived from the rat fetal cortex, we defined the effects of oxygen and glucose deprivation on NPY expression, using BDNF‐induced production of NPY as a functional criterion. NPY neurons exhibited a differential susceptibility to oxygen and glucose deprivation. Although the neurons could withstand oxygen deprivation for 16 hr, they were dramatically damaged by 8 hr of glucose deprivation and by 1–4 hr of deprivation of both oxygen and glucose (N+Glu−). One‐hour exposure to N+Glu− led to a transient inhibition (≈50%) of NPY production manifesting within 24 hr and recovering by 5 days thereafter, a 2‐hr exposure to N+Glu− led to a sustained inhibition (50–75%) manifesting 1–5 days thereafter, and a 4‐hr exposure to N+Glu− led to a total irreversible suppression of BDNF‐induced production of NPY manifesting within 24 hr and lasting 8 days after re‐supply of oxygen and glucose. Moreover, 1‐hr exposure to N+Glu− led to a substantial and 4‐hr exposure led to a total disappearance of immunostaining for MAP‐2 and NPY but not for GFAP; indicating that neurons are the primary cell‐type damaged by oxygen‐glucose deprivation. Analysis of cell viability (LDH, MTT) indicated that progressive changes in cell integrity take place during the 4‐hr exposure to N+Glu− followed by massive cell death 24 hr thereafter. Thus, we defined a culture system that can serve as a model to study mechanisms by which ischemic insult leads to suppression and eventually death of NPY neurons. Importantly, changes in NPY neurons can be integrated into the overall scheme of ischemic injury in the perinatal brain. © 2002 Wiley‐Liss, Inc.