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Coexpression of thyroid hormone receptor isoforms in mouse oligodendrocytes
Author(s) -
Bury Fabienne,
Carré JeanLuc,
Vega Sonia,
Ghandour M. Said,
RodriguezPeña Angeles,
Langley Keith,
Sarliève Louis L.
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10111
Subject(s) - immunocytochemistry , gene isoform , biology , microbiology and biotechnology , oligodendrocyte , receptor , antibody , thyroid hormone receptor , epitope , endocrinology , medicine , thyroid , gene , myelin , immunology , central nervous system , biochemistry
Double and triple immunocytochemistry with stage‐specific markers and specific antireceptor antibodies was used to study expression of nuclear thyroid hormone receptor (TR) isoforms in cultured mouse oligodendrocytes. To evaluate the coexpression of each TR isoform, antibodies were raised in rabbits and mice against specific regions of α1‐TR and α2‐TR common to both α isoforms and β1‐TR. Their specificities were assessed by Western blotting and by immunocytochemistry on rat hepatocytes. Oligodendrocyte subpopulations were found to coexpress the α‐ and β1‐TR epitopes at defined developmental stages. Both α‐ and β1‐TR isoforms are colocalized in oligodendrocytes during an early stage identified by the marker OL‐1, before 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase is expressed. Expression of β1‐TR varies during maturation, and that of α‐TR decreases during terminal maturation. © 2002 Wiley‐Liss, Inc.