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Serotonin 2C receptor knockout mice: Autoradiographic analysis of multiple serotonin receptors
Author(s) -
LópezGiménez Juan F.,
Tecott Laurence H.,
Palacios José M.,
Mengod Guadalupe,
Vilaró M. Teresa
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10072
Subject(s) - serotonin , serotonergic , receptor , 5 ht receptor , medicine , endocrinology , serotonin transporter , biology , knockout mouse , chemistry
Quantitative receptor autoradiography was used to study possible alterations of the densities of multiple serotonin (5‐HT) receptor subtypes and of serotonin transporter in the brain of 5‐HT 2C receptor knockout mice. The radioligands employed were [ 3 H]citalopram, [ 3 H]WAY100,635, [ 3 H]8‐OH‐DPAT, [ 3 H]GR125743, [ 3 H]sumatriptan, [ 3 H]MDL100,907, [ 125 I](±)DOI, [ 3 H]mesulergine, [ 3 H]5‐HT, [ 3 H]GR113808, and [ 3 H]5‐CT. As expected, radioligands that label 5‐HT 2C receptors showed a complete absence of labeling in mutant mice choroid plexus and significantly reduced densities in other brain regions expressing 5‐HT 2C receptors. With the rest of the radioligands, no significant alterations in the densities of labeled sites were found in any brain region. In situ hybridization showed no changes in 5‐HT 2A receptor and serotonin transporter mRNA levels, whereas 5‐HT 2C receptor mRNA levels were reduced in certain brain regions. The present results indicate that the mouse serotonergic system does not exhibit compensatory up‐ or down‐regulation of the majority of its components (serotonin transporter and most 5‐HT receptor subtypes) in response to the absence of 5‐HT 2C receptors. © 2002 Wiley‐Liss, Inc.