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Local uptake of 14 C‐labeled acetate and butyrate in rat brain in vivo during spreading cortical depression
Author(s) -
Dienel Gerald A.,
Liu Kenian,
Cruz Nancy F.
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10063
Subject(s) - cortical spreading depression , butyrate , cerebral cortex , cortex (anatomy) , butyric acid , chemistry , astrocyte , metabolism , in vivo , endocrinology , medicine , kynurenic acid , acetic acid , biochemistry , biology , central nervous system , glutamate receptor , neuroscience , receptor , migraine , fermentation , microbiology and biotechnology
Spreading depression severely disrupts ion homeostasis, causes sensory neglect and motor impairment, and is associated with stroke and migraine. Glucose utilization (CMR glc ) and lactate production rise during spreading depression, but the metabolic changes in different brain cell types are unknown. Uptake of 14 C‐labeled compounds known to be preferentially metabolized by the glial tricarboxylic acid cycle was, therefore, examined during unilateral KCl‐induced spreading cortical depression in conscious, normoxic rats. [ 14 C]Metabolites derived from [ 14 C]butyrate in K + ‐treated tissue rose 21% compared to that of untreated contralateral control cortex, whereas incorporation of H 14 CO 3 into metabolites in K + ‐treated tissue was reduced to 86% of control. Autoradiographic analysis showed that laminar labeling of cerebral cortex by both 14 C‐labeled acetate and butyrate was elevated heterogeneously throughout cortex by an average of 23%; the increase was greatest (∼40%) in tissue adjacent to the K + application site. Local uptake of acetate, butyrate, and deoxyglucose showed similar patterns, and monocarboxylic acid uptake was highest in the structures in which apparent loss of labeled metabolites of [6‐ 14 C]glucose was greatest. Enhancement of net uptake of acetate and butyrate in cerebral cortex during spreading depression is tentatively ascribed to increased astrocyte metabolism. © 2001 Wiley‐Liss, Inc.