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Differential expression of glutamate dehydrogenase in cultured neurons and astrocytes from mouse cerebellum and cerebral cortex
Author(s) -
Zaganas Ioannis,
Waagepetersen Helle S.,
Georgopoulos Panagiotis,
Sonnewald Ursula,
Plaitakis Andreas,
Schousboe Arne
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10058
Subject(s) - glutamatergic , glutamate receptor , cerebellum , glutamate dehydrogenase , cerebral cortex , gabaergic , biology , neuroscience , glutamate decarboxylase , astrocyte , microbiology and biotechnology , cell type , biochemistry , enzyme , cell , central nervous system , inhibitory postsynaptic potential , receptor
Glutamate dehydrogenase (GDH) specific activities, kinetic properties and allosteric regulation were studied in extracts from cultured neurons and astrocytes prepared from mouse cerebral cortex and cerebellum. Considerable differences were observed in the specific activity of the enzyme among the different cell types with astrocytes expressing the highest GDH activity. This may reflect the functional importance of these cells in glutamate uptake and metabolism. Among the neurons, the glutamatergic cerebellar granule cells showed a GDH specific activity that was 60% higher ( P < 0.01) than that of the GABAergic cerebral cortical neurons. Also, the K m for ammonia was 1.7‐fold higher in the cortical neurons than in the other cell types. These findings may reflect a particular need for the glutamatergic granule cells to synthesize glutamate via the GDH pathway. No differences were observed among the different cell types with regard to the allosteric properties of GDH expressed by these cells. © 2001 Wiley‐Liss, Inc.