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Myelin phagocytosis and remyelination of macrophage‐enriched central nervous system aggregate cultures
Author(s) -
Copelman Cheryl A.,
Diemel Lara T.,
Gveric Djordje,
Gregson Norman A.,
Cuzner M. Louise
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10026
Subject(s) - remyelination , myelin , myelin basic protein , microglia , phagocytosis , macrophage , microbiology and biotechnology , chemistry , peptide , multiple sclerosis , immunology , central nervous system , biology , inflammation , neuroscience , biochemistry , in vitro
An increased level of myelin basic protein (MBP) degradation peptide 80–89, representative of myelin breakdown, is detected in myelinating foetal rat brain aggregate cultures supplemented with peritoneal macrophages at a time coinciding with the onset of myelination. During the period of myelination, the proportion of activated macrophages/microglia in the aggregates decreases, accompanied by a reduction in the content of MBP degradation products. During the recovery period following a demyelinating episode, the rate of MBP synthesis in antibody‐treated standard aggregates was greater than in their medium controls. However, the rate of MBP accumulation was not as efficient in macrophage‐enriched aggregates and was associated with persistently raised MBP peptide levels. Thus, as occurs in multiple sclerosis lesions, attempts at remyelination appear to be counterbalanced by macrophage‐mediated demyelination, with the continued presence of degraded myelin rendering a local environment that is not fully conducive to remyelination. J Neurosci. Res. 66:1173–1178, 2001. © 2001 Wiley‐Liss, Inc.