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Neurogenesis of heterotopic gray matter in the brain of the microcephalic mouse
Author(s) -
Sun XueZhi,
Takahashi Sentaro,
Fukui Yoshihiro,
Hisano Setsuji,
Kubota Yoshihisa,
Sato Hiroshi,
Inouye Minoru
Publication year - 2001
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.10018
Subject(s) - neuroblast , glial fibrillary acidic protein , neurogenesis , biology , gfap stain , pathology , astrocyte , parvalbumin , heterotopia (medicine) , cerebrum , immunostaining , bromodeoxyuridine , anatomy , chemistry , microbiology and biotechnology , neuroscience , immunohistochemistry , central nervous system , medicine , immunology
Neurogenesis of heterotopic gray matter in the brain of the microcephalic mouse prenatally exposed to X‐rays at embryonic day 13 (E13) was studied immunohistochemically. Bromodeoxyuridine (BrdU) as a marker to label the migrating position of neuroblasts generated at various embryonic stages showed that no “inside‐out” pattern of neuronal migration occurred in the heterotopic cell mass similar to that seen in the laminated cortex. Further results in which midkind (MK) immunoreactive radial glial fibers did not appear in the heterotopic cell mass demonstrated that heterotopia formed in the absence of radial glia system. Different types of cells (pyramidal and non‐pyramidal neurons) in the heterotopic cell mass were identified with immunoreactivity for anti‐parvalbumin and anti‐calbindin D‐28K antibodies in addition to current histological methods. Two major types of neurons were mixed together with random distribution in the heterotopic cell mass. This finding indicates that irradiation might have no selective effects on the precursors of pyramidal and non‐pyramidal neurons. Moreover, anti‐glial fibrillary acidic protein (GFAP) immunostaining showed that numerous astrocytes were present in the heterotopic cell mass. The fact that astrocytes appeared in the heterotopia without the transition from classic radial glial cells to astrocytes suggests that astrocytes might be generated directly from a separate astroglial precursor. J Neurosci. Res. 66:1083–1093, 2001. © 2001 Wiley‐Liss, Inc.