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Association between cycle threshold ( C t ) values and clinical and laboratory data in inpatients with COVID‐19 and asymptomatic health workers
Author(s) -
RamirezHinojosa Juan Pablo,
RodriguezSanchez Yunuen,
RomeroGonzalez Angel Kaleb,
ChavezGutierrez Marisol,
GonzalezArenas Nelly Raquel,
IbarraArce Aurora,
ArroyoEscalante Sara,
ZavaletaVilla Beatriz,
LeonJuarez Moises,
CruzHolguin Victor Javier,
Espinosa de los MonterosPerez Luz Elena,
OlivoDiaz Angelica,
HernandezCastro Rigoberto,
SuarezRoa Lourdes,
PradoCalleros Hector,
SierraMartinez Octavio,
AvilaRamirez Guillermina,
Flisser Ana,
Maravilla Pablo,
RomeroValdovinos Mirza
Publication year - 2021
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.27170
Subject(s) - asymptomatic , medicine , covid-19 , saliva , real time polymerase chain reaction , virology , viral load , immunology , human immunodeficiency virus (hiv) , biology , gene , disease , infectious disease (medical specialty) , genetics
In‐house assays for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR), are feasible alternatives, particularly in developing countries. Cycle threshold ( C t ) values obtained by qRT‐PCR were compared with clinical and laboratory data from saliva of inpatients with COVID‐19 and asymptomatic health workers (AHW) were studied. Saliva specimens from 58 inpatients confirmed by qRT‐PCR for SARS‐CoV‐2 using nasopharyngeal specimens, and 105 AHW were studied by qRT‐PCR using three sets of primers for the N ( N 1, N 2, and N 3) gene of SARS‐CoV‐2, according to the CDC Diagnostic Panel protocol, showing a positivity of 88% for inpatients and 8% for AHW. Bivariate analysis revealed an association between C t < 38.0 values for N 2 and mechanical ventilation assistance among patients ( p = .013). In addition, values of aspartate‐transaminase, lactate dehydrogenase, and ferritin showed significant correlations with C t values of N 1 and N 3 genes in inpatients. Therefore, our results show that C t values correlate with some relevant clinical data for inpatients with COVID‐19.