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Evaluation of the diagnostic performance of nine commercial RT‐PCR kits for the detection of SARS‐CoV‐2 in Colombia
Author(s) -
Hernández Carolina,
Florez Carolina,
Castañeda Sergio,
Ballesteros Nathalia,
Martínez David,
Castillo Adriana,
Muñoz Marina,
Gomez Sergio,
Rico Angelica,
Pardo Liseth,
PanizMondolfi Alberto,
Ramírez Juan David
Publication year - 2021
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.27051
Subject(s) - covid-19 , virology , polymerase chain reaction , molecular diagnostics , pandemic , diagnostic test , reverse transcription polymerase chain reaction , medicine , coronavirus , biology , gene , genetics , pathology , emergency medicine , gene expression , infectious disease (medical specialty) , disease , outbreak
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic has led to the design and development of multiple reverse‐transcription polymerase chain reaction kits aimed to facilitate the rapid scale‐up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS‐CoV‐2 genetic variability and its potential impact in current molecular diagnostic assays.