Premium
Evolutionary analysis of SARS‐CoV‐2 spike protein for its different clades
Author(s) -
Pereson Matías J.,
Flichman Diego M.,
Martínez Alfredo P.,
Baré Patricia,
Garcia Gabriel H.,
Di Lello Federico A.
Publication year - 2021
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.26834
Subject(s) - clade , virology , covid-19 , spike protein , spike (software development) , biology , sars virus , coronavirus , pandemic , betacoronavirus , evolutionary biology , genetics , phylogenetics , computational biology , gene , medicine , computer science , outbreak , infectious disease (medical specialty) , disease , pathology , software engineering
The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has become the main target for antiviral and vaccine development. Despite its relevance, e information is scarse about its evolutionary traces. The aim of this study was to investigate the diversification patterns of the spike for each clade of SARS‐CoV‐2 through different approaches. Two thousand and one hundred sequences representing the seven clades of the SARS‐CoV‐2 were included. Patterns of genetic diversifications and nucleotide evolutionary rate were estimated for the spike genomic region. The haplotype networks showed a star shape, where multiple haplotypes with few nucleotide differences diverge from a common ancestor. Four hundred seventy‐nine different haplotypes were defined in the seven analyzed clades. The main haplotype, named Hap‐1, was the most frequent for clades G (54%), GH (54%), and GR (56%) and a different haplotype (named Hap‐252) was the most important for clades L (63.3%), O (39.7%), S (51.7%), and V (70%). The evolutionary rate for the spike protein was estimated as 1.08 × 10 −3 nucleotide substitutions/site/year. Moreover, the nucleotide evolutionary rate after nine months of the pandemic was similar for each clade. In conclusion, the present evolutionary analysis is relevant as the spike protein of SARS‐CoV‐2 is the target for most therapeutic candidates; besides, changes in this protein could have consequences on viral transmission, response to antivirals and efficacy of vaccines. Moreover, the evolutionary characterization of clades improves knowledge of SARS‐CoV‐2 and deserves to be assessed in more detail as re‐infection by different phylogenetic clades has been reported.