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Dengue RNA detection and seroprevalence in blood donors during an outbreak in the São Paulo State, Brazil, 2016
Author(s) -
Slavov Svetoslav N.,
Santos Elaine V.,
Hespanhol Marta R.,
Rodrigues Evandra S.,
Haddad Rodrigo,
Ubiali Eugênia M. A.,
Covas Dimas T.,
Kashima Simone
Publication year - 2021
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.26619
Subject(s) - dengue virus , virology , dengue fever , seroprevalence , outbreak , population , biology , viremia , antibody , serology , virus , immunology , medicine , environmental health
Most dengue virus (DENV) infections remain asymptomatic. This increases the risk of DENV transfusion transmission (TT‐DENV) during outbreaks. We evaluated DENV viremia in 8475 blood donations assembled in minipools for the presence of DENV RNA. The tested samples were obtained between February and May, 2016, during a large DENV outbreak in Ribeirão Preto city, northeast region of the São Paulo State, Brazil. The DENV RNA + samples were serotyped and screened for DENV NS1. We also tested a significant number of plasma samples ( n  = 372) to estimate the DENV seroprevalence among blood donors in the region. We detected three DENV RNA + samples in the tested blood donations ( n  = 3/8475, 0.04%). From these, two samples were further serotyped as DENV‐1 and one sample as DENV‐2. All DENV RNA positive samples were negative for anti‐DENV IgG, indicating the presence of primary acute infection. Moreover, two of the DENV RNA + samples were also NS1 antigen positive (antigenemia). The anti‐DENV IgG seroprevalence among blood donor population was 50.8% ( n  = 189/372). Our results are in accordance with the presence of DENV primary infection in blood donors which can lead to transfusion transmission of the infection to recipients. Measures to exclude such donors should be adopted to prevent TT‐DENV.

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